Lifetime-resolved photon-correlation Fourier spectroscopy

Time-resolved methods in biophysics. 3. Fluorescence lifetime correlation spectroscopy.

We present a thorough introduction into the recently developed fluorescence lifetime correlation spectroscopy (FLCS). The theoretical basis of FLCS is explained, and the method is applied to the study of a dynamic transition between two fluorescence lifetime states in a dye-protein complex.

Photon Correlation Spectroscopy

FLCS – Fluorescence Lifetime Correlation Spectroscopy

The fusion of Time-Correlated Single Photon Counting and Fluorescence Correlation Spectroscopy, called Fluorescence Lifetime Correlation Spectroscopy (FLCS), is a method that uses picosecond time-resolved fluorescence detection for separating different FCS-contributions. The main emphasis is on the word separating. FLCS does not involve fitting of a complicated model equation to a complicated a...

EMCCD-based spectrally resolved fluorescence correlation spectroscopy.

We present an implementation of fluorescence correlation spectroscopy with spectrally resolved detection based on a combined commercial confocal laser scanning/fluorescence correlation spectroscopy microscope. We have replaced the conventional detection scheme by a prism-based spectrometer and an electron-multiplying charge-coupled device camera used to record the photons. This allows us to rea...

Photon-correlation Fourier spectroscopy of the trion fluorescence in thick-shell CdSe/CdS nanocrystals

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